M4308
Sigma
Monoclonal Anti-MDM2 antibody produced in mouse
clone SMP14, ascites fluid
| MDL number: | MFCD03792424 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH. |
| Specificity | Detects the MDM2 protein alone or in complex with E2F1, p53, and retinoblastoma proteins. A slight cross-reactivity is observed with cytokeratins 6, 14, and 16. |
Properties
| antibody form | ascites fluid |
| clone | SMP14 (monoclonal) |
| mol wt | antigen mol wt ~90 kDa (additional band of approx. 55 kDa) |
| contains | 15 mM sodium azide |
| species reactivity | human, rat, mouse (weak) |
| application(s) | immunoblotting: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2 |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... MDM2(4193) |
| rat ... Mdm2(246362) | |
| mouse ... Mdm2(17246) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Picksley, S.M., Immunochemical analysis of the interaction of p53 with MDM2; fine mapping of the MDM2 binding site on p53 using synthetic peptides. Oncogene 9, 2523, (1994) Abstract |
| Hickman, E.S., et al., Cells expressing HPV16 E7 continue cell cycle progression following DNA damage induced p53 activation. Oncogene 9, 2177-2181, (1994) Abstract | |
| Martin, K., et al., Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein. Nature 375, 691-694, (1995) Abstract | |
| Yao, A., et al., Stabilization of p53 is a novel mechanism for proapoptotic function of NF-kappaB. J. Biol. Chem. 279, 27549-27559, (2004) Abstract |
